Thursday, May 17, 2012

The frequency of discomfort can be reduced by effective botanicalslimming

Taking effective botanical slimming is able to reduce fat of obese people.   (Sac workers Difference between the two Sac workers digested fragments for the sticky side, and Eco1CRI the digested fragments of flat HSL gene inserted between the end) and Hind III son and a 697 "+53 by the sequence, the construct containing HSL Due to the promoter sequence of the luciferase expression plasmid pGL3-HSL-Lue, referred to as 697-Luc. In this experiment, HSL gene promoter activity in traditional Chinese medicine extract. 'Mutual , Z-Chu said mouth M produced' consultative Fourth, cell lines.
effective botanical slimming can improve memory of obese people.  Luciferase expression plasmid 697-Luc and 3 Fg in the neomycin resistance Gene expression plasmid pcDNA3 (+) ratio of 3: January stick even after 8 fluid, be careful to stick together A, B, two liquid For 20 min at room temperature, add the above have been ready for 24 holes of the growth of mouse 3T3-L 1 preadipocytes Board, each hole 0 1 ml, a total of 15 holes at 370C incubator transfection 5h, the replacement of complete DMEM: F12 (i: l) Medium continued for 48 h. Cells were collected, re-seeded in 60 mm culture dish, and add 400} g / Ml.
The traditional view is that in the mobilization of fat in fat cells Hormone-sensitive lipase (HSL) play a decisive role, which is the rate-limiting enzyme of lipolysis.  effectivebotanical slimming can relieve low mood.  The nature of obesity is the accumulation of body fat [Sichuan. Lipolysis process as the role of weight loss drugs one of the targets are Received extensive attention, stimulate lipolysis to reduce fat cells store fat is one of the direction of the obesity drug development [: 1l '. As an important lipase in the lipolysis process.
 Before the fat Increase in fat cell lysate luciferase expression in mouse 3T3-L1 fat cells 2h after drug: Forsythia, Arisaema, raw hawthorn, Atractylodes, forsythia, Ge Po Chuan Shao, Adenophorae, Sichuan pepper, triangular, Luo Han fruit, Platycodon, honeysuckle, jasmine, mulberry, licorice root, Cyperus, melon skin, seaweed and Hui m ((P (0 05); Caulis, Oolong tea, from generation to generation flower, Longjing tea luciferase expression decreased ((P <0 05) with the concentration of 25mg/ml.  effective botanical slimming can help heavy smokers.
 For 24 hours after the fat cell lysate luciferase expression increased in mouse 3T3-L1 Drugs added: Cyperus rotundus, Magnolia, from generation to generation flowers, mulberry leaf, provoke m, licorice root, psyllium, Arisaema and jasmine Flowers ((P << 0.05), suggesting that under this condition, these drugs may be possible through the excitement of HSL gene transcription and to promote Into the lipolysis; and Vaccaria Polygala, oolong tea, forsythia, Campanulaceae, Sichuan pepper, night cross the Po of the vine, repair, Longjing tea and seaweed luciferase expression to reduce the ((P <0 05). To prevent obesity, people need to take some effective botanical slimming properly.

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